Under new ownership and management as a woman-owned and veteran small business.

Over 300 different modifications available for oligos.

Any DNA or RNA polymer are 30 percent off for the month of October, 2015 if ordered on the web site.

HPLC Purification is 30 percent off for October if ordered on the web site.

Please excuse our construction on the web as we strive to make improvements everyday.

Midland is a leader in the synthesis of DNA and RNA oligonucleotides for over 35 years. We pioneered the use of MALDI-TOF mass spectrometry for quality assurance of oligonucleotides, a technique that has become the gold standard for the industry. Guided by our commitment to quality we have designed and built DNA synthesizers that produce oligonucleotides of higher sequence fidelity than any instruments commercially available today.

Midland oligos are often used for gene synthesis in the following way. Two sets of 40-mers are synthesized, each set comprising one of the two strands of the desired gene. Each oligo overlaps a 20-base region of two oligos in the complementary set. The oligos are all combined in approximately equal amounts and several cycles of amplification by PCR are performed. Finally two oligos corresponding to the 5’-ends of each of the strands are added and several more cycles of PCR are run. The products are purified by electrophoresis and the product of the desired size is collected.

The process is used for example in the technique called genetic immunization (De-chu Tang, Michael DeVit & Stephen A. Johnston, NATURE 536, 152-154 (1992)). A synthetic gene is coated onto gold microspheres and introduced into a small animal with a gene gun. The animal produces antibodies to the protein encoded by the synthesized gene. This obviates the need to purify the antigenic protein.

Another outgrowth of our focus on the quality of our synthesis chemistry is the ability to modify oligonucleotides. Today we offer more than 200 modifications, including fluorophores, quenchers, backbone modifications, degeneracies, dual-labeled probes, and minor nucleotides. To view the complete alphabetical listing, click here. While several modifications can be ordered directly from this website, many pose technical challenges that we will need to discuss with you before we proceed to manufacture. Evidence of success in the modification is provided by the printout of the MALDI-TOF mass spectrum of every modified oligo.

We continue to offer all of the popular purification modalities, such as HPLC and PAGE purification. However, synthesis technology has advanced sufficiently that for many applications purification that was essential a few years ago is no longer necessary. Thus, for example, genes are constructed with GF grade (desalted) oligonucleotides without the need for further purification.

We continue to offer a wide range of polynucleotides, including duplexed polynucleotides and template/primer combinations. Click for lists of DNA Polynucleotides, RNA Polynucleotides, or Template/Primers used to distinguish reverse transcriptase from DNA polymerase activities.

We also sell and offer information on such popular products such as:

RNA Polymers for RNA Synthesis and other experiments

DNA Polymers for DNA Synthesis and other experiments

Amino-Modified Oligos

Thiol-Modified Oligos

Please inquire about LNAs at – sandy@oligos.com

OVER 300 different modifications available for DNA, RNA, and LNA synthesis

And more… Our List of Overseas Distributors:

India – GenxBio Health Sciences Pvt. Ltd.

Jordan – Burjouan Medical Laboratory Supplies We welcome your product inquiries and technical questions at (800) 247-8766 or (432) 694-7950, where a real person, not a recording, will greet you! You may also send inquiries to info@oligos.com.

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