Summary: Antisense oligonucleotides are playing large roles in disease prevention.
Throughout the course of oligonucleotide history, major improvements and discoveries have been made in molecular biology that have allowed antisense therapy to thrive. This has allowed researchers to thrive in understanding diseases at the molecular level, which has essentially facilitated the development of new drugs based on a rational design. This has opened up thousands of possibilities and is only the foundation of what is yet to come.
Antisense therapy is the application of rational design based on the concept of oligonucleotide chemistry. Zamenick and Stephenson were the first to illustrate this idea and present it to the world when they demonstrated that oligonucleotides could inhibit the replication of the Rous sarcoma in the body´s cellular system. Because of this principle, it allowed researchers to approach antisense in a simple way: inhibit the expression of a targeted gene at the RNA level and attaining complimentary oligonucleotides will follow. These are what are called antisense oligonucleotides, which block the expression of the protein that is encoded by the target RNA or synthetic DNA. If the inhibited proteins are the ones related to disease, then those oligonucleotides become a new class of drugs.
With the combination of both RNA and DNA synthesis in the cellular body, there are several reactions that occur that allow scientists to gain insightful data on to how the antisense oligonucleotide can overall affect a disease. This continues to be researched as antisense therapy continues to make a mark in the medical field, with impending breakthroughs just waiting to occur.
Bio: Oligonucleotides, dual labeled probes, polynucleotide samples, and more are available from the Midland Certified Reagent Company. For more details, visit them online today.
Summary: To distinguish one member from another in the pool of oligos, a tagging system has been created.
Over the last ten years, there has been significant improvement in the development of compound libraries for the discovery of molecules with new binding properties. The basic premise is to ultimately shorten the effort and time associated with the search of drugs leads by creating a large combination of molecules that are tested against a certain target for a specified activity. However, there is a problem with this technique as that there lacks a proper strategy to determine the identity of the active molecules from the mess of the original pool. To solve this, researchers have developed a system that utilizes either tagged or untagged labels.
The use of these tags have established a beneficial relationship between each single member of the library and the tags itself with gives it a unique identity. Even through the process of synthetic DNA playing a role in presenting itself as a different member, these powerful tag technologies have become important tools to separate themselves from the pack. Additionally, researchers have discovered flexible and versatile ways to apply these tags to multiple areas of molecular biology, therefore expanding the real of possibility.
There is a certain strategy that makes use of modified oligos that´s called Selective Enhancement of Ligands by Exponential Enrichment, or SELEX for short. What this technique does is executes multiple cycles of selection and amplification, which results in competition between the molecules that are deemed suitable from the huge population in the pool. Essentially, the pool is separated by individual members to appropriate them to a given task (usually a binding or catalytic task).
Bio: The Midland Certified Reagent Company is your number one supplier of oligos, samples used for RNA synthesis, and dual-labeled probes. For more information, visit them online today.
Oligonucleotide synthesis is essentially the synthesis of nucleic acid fragments that results in defined chemical structures with purities of different sizes. When you´re looking for the most effective modified product for your oligo synthesis, you´re going to want to narrow down the companies that you´re going to work with by high quality services that they offer. Here are some key components that you´re going to want to keep in mind when shopping around.
Quality is obviously the main concern that you´re going to have because the only way that you can validate what they claim is through user reviews or testimonials. You´re not going to have a physical sample that you get to take an in-depth look at, rather an assortment of pictures put together by the company.
Be sure that the company is specific in their details to ensure that you get exactly what you want. For example, if they´re offering single stranded or double-stranded DNA, be sure that they give you the exact number of bases as they state. Additionally, check to see if they are analyzed and quantified by both mass spectrometry as well as UV spectrophotometry. There are also certain standards that should be met by the manufacturer such as specifications and formats of the oligos.
Oligonucleotide manufacturers that deal with such delicacy should have an informative group of customer service representatives. If you´re unsure about the purchase of a product or want to validate what you´re receiving, speak with their customer service representatives and ask them specific questions such as: the research grade, their formats, packaging, etc.